AnEcoRI RFLP in humanAPC gene

MspI RFLP in the human fumarylacetoacetate hydrolase (FAH) gene.

RFLP Analysis of Beta‐Lactoglobulin Gene in Swamp and Murrah Buffaloes Using a Single Restriction Enzyme

An attempt has been made to analyze the distribution of the beta-lactoglobulin genotype in swamp buffaloes and Murrah buffaloes utilizing polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Blood samples were taken from 50 swamp buffaloes and 50 Murrah buffaloes. The DNA was extracted by the phenol-chloroform method. The PCR-RFLP was performed using beta-lactoglobulin ...

RFLP Molecular Analysis of the Urokinase-Type Plasminogen Activator Gene.

Several cell biological studies have shown that the invasiveness of a variety of tumors depend on the regulated expression of proteolytic enzymes that degrade the surrounding extracellular matrix and dissociate cell-cell and/or cell-matrix attachments. One such enzyme, the serine protease urokinase-type plasminogen activator (uPA), converts enzymatically inactive plasminogen into the widely act...

A TaqI RFLP for the human arylsulfatase A gene.

TaqI RFLP polymorphism of the ovine complement component C4 gene.

Protocol: Reactions were performed as described (2) using 1 ng of DNA and 100 pmoles of each primer. DNA was amplified for 20 cycles using a Perkin Elmer Cetus DNA Thermal Cycler: denaturation, 94°C, 1 min; annealing, 60°C, 1 min; and extension, 70°C, 1.5 min. Ten /il of the reaction was analyzed directly on a 2.5 % NuSieve GTG agarose gel run in Tns-borate buffer. The amplified DNA fragments v...